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Combination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energyCombination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energy Shockwaves on the Corpus Cavernosum in a Rat Model of Post-pro SCIE SCOPUS

Title
Combination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energyCombination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energy Shockwaves on the Corpus Cavernosum in a Rat Model of Post-pro
Authors
Jeon, S.HShrestha, K.RKim, R.YJung, A.RPark, Y.HKwon, OKim, G.EKim, S.HKim, K.HLee, J.Y.
Date Issued
2016-02
Publisher
Elsevier
Abstract
OBJECTIVE To investigate combined therapeutic efficacy of human adipose-derived stem cells (h-ADSCs) application on injured cavernous nerve and low-energy shockwave therapy (SWT) on the corpus cavernosum in a rat model of post-prostatectomy erectile dysfunction. MATERIALS AND METHODS Rats were randomly divided into 5 groups: control, bilateral cavernous nerve injury (BCNI), adipose-derived stem cell (ADSC) (BCNI group with h-ADSCs on the cavernous nerve), SWT (BCNI group with low-energy SWT on the corpus cavernosum), and ADSC/SWT (BCNI group with a combination of h-ADSCs and low-energy SWT). After 4 weeks, erectile function was assessed using intracavernosal pressure. The cavernous nerves and penile tissue were evaluated through immunostaining, Western blotting, and a cyclic guanosine monophosphate assay. RESULTS ADSC/SWT significantly improved intracavernosal pressure compared to the other experimental group. ADSC had significantly increased beta-III tubulin expression of the cavernous nerve, and SWT had a markedly enhanced vascular endothelial growth factor expression in corpus cavernosum. The ADSC/SWT group had a significantly increased in alpha smooth muscle actin content (P < .05), neural nitric oxide synthase (nNOS) of the dorsal penile nerve (P < .05), endothelial nitric oxide synthase (eNOS) protein expression (P < .05), and cyclic guanosine monophosphate level (P < .05) compared to the ADSC or SWT alone group. In addition, ADSC/SWT reduces the apoptotic index in the corpus cavernosum. CONCLUSION In this study, h-ADSCs showed an effect on the recovery of injured cavernous nerve and low-energy SWT improved angiogenesis in the corpus cavernosum. The h-ADSCs combined with low-energy SWT showed beneficial effect on the recovery of erectile function in postprostatectomy erectile dysfunction. (C) 2016 Elsevier Inc.
URI
https://oasis.postech.ac.kr/handle/2014.oak/35402
DOI
10.1016/J.UROLOGY.2015.10.021
ISSN
0090-4295
Article Type
Article
Citation
UROLOGY, vol. 88, no. 15, page. 1010 - 1019, 2016-02
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김기현KIM, KI HEAN
Dept of Mechanical Enginrg
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