DC Field | Value | Language |
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dc.contributor.author | Jeon, S.H | - |
dc.contributor.author | Shrestha, K.R | - |
dc.contributor.author | Kim, R.Y | - |
dc.contributor.author | Jung, A.R | - |
dc.contributor.author | Park, Y.H | - |
dc.contributor.author | Kwon, O | - |
dc.contributor.author | Kim, G.E | - |
dc.contributor.author | Kim, S.H | - |
dc.contributor.author | Kim, K.H | - |
dc.contributor.author | Lee, J.Y. | - |
dc.date.accessioned | 2017-07-19T12:11:30Z | - |
dc.date.available | 2017-07-19T12:11:30Z | - |
dc.date.created | 2016-01-08 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 0090-4295 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/35402 | - |
dc.description.abstract | OBJECTIVE To investigate combined therapeutic efficacy of human adipose-derived stem cells (h-ADSCs) application on injured cavernous nerve and low-energy shockwave therapy (SWT) on the corpus cavernosum in a rat model of post-prostatectomy erectile dysfunction. MATERIALS AND METHODS Rats were randomly divided into 5 groups: control, bilateral cavernous nerve injury (BCNI), adipose-derived stem cell (ADSC) (BCNI group with h-ADSCs on the cavernous nerve), SWT (BCNI group with low-energy SWT on the corpus cavernosum), and ADSC/SWT (BCNI group with a combination of h-ADSCs and low-energy SWT). After 4 weeks, erectile function was assessed using intracavernosal pressure. The cavernous nerves and penile tissue were evaluated through immunostaining, Western blotting, and a cyclic guanosine monophosphate assay. RESULTS ADSC/SWT significantly improved intracavernosal pressure compared to the other experimental group. ADSC had significantly increased beta-III tubulin expression of the cavernous nerve, and SWT had a markedly enhanced vascular endothelial growth factor expression in corpus cavernosum. The ADSC/SWT group had a significantly increased in alpha smooth muscle actin content (P < .05), neural nitric oxide synthase (nNOS) of the dorsal penile nerve (P < .05), endothelial nitric oxide synthase (eNOS) protein expression (P < .05), and cyclic guanosine monophosphate level (P < .05) compared to the ADSC or SWT alone group. In addition, ADSC/SWT reduces the apoptotic index in the corpus cavernosum. CONCLUSION In this study, h-ADSCs showed an effect on the recovery of injured cavernous nerve and low-energy SWT improved angiogenesis in the corpus cavernosum. The h-ADSCs combined with low-energy SWT showed beneficial effect on the recovery of erectile function in postprostatectomy erectile dysfunction. (C) 2016 Elsevier Inc. | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | UROLOGY | - |
dc.title | Combination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energyCombination Therapy Using Human Adipose-derived Stem Cells on the Cavernous Nerve and Low-energy Shockwaves on the Corpus Cavernosum in a Rat Model of Post-pro | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/J.UROLOGY.2015.10.021 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | UROLOGY, v.88, no.15, pp.1010 - 1019 | - |
dc.identifier.wosid | 000373464600058 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.endPage | 1019 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 1010 | - |
dc.citation.title | UROLOGY | - |
dc.citation.volume | 88 | - |
dc.contributor.affiliatedAuthor | Kim, K.H | - |
dc.identifier.scopusid | 2-s2.0-84980007067 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 15 | - |
dc.description.scptc | 9 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | WAVE THERAPY | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | RECOVERY | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | PROSTATECTOMY | - |
dc.subject.keywordPlus | PROGENITORS | - |
dc.subject.keywordPlus | VIVO | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
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