Mutations of the multi-drug resistance-associated protein ABC transporter gene 5 result in reduction of phytic acid in rice seeds
SCIE
SCOPUS
- Title
- Mutations of the multi-drug resistance-associated protein ABC transporter gene 5 result in reduction of phytic acid in rice seeds
- Authors
- Xu, XH; Zhao, HJ; Liu, QL; Frank, T; Engel, KH; An, GH; Shu, QY
- Date Issued
- 2009-06
- Publisher
- SPRINGER
- Abstract
- Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) is important to the nutritional quality of cereal and legume seeds. PA and its salts with micronutrient cations, such as iron and zinc, cannot be digested by humans and non-ruminant animals, and hence may affect food/feed nutritional value and cause P pollution of groundwater from animal waste. We previously developed a set of low phytic acid (LPA) rice mutant lines with the aim of increasing the nutritional quality of rice. Two of these lines, Os-lpa-XS110-2 (homozygous non-lethal) Os-lpa-XS110-3 (homozygous lethal), contain two mutant alleles of a LPA gene (hereafter XS-lpa2-1 and XS-lpa2-2, respectively). In this study, we mapped the XS-lpa2-1 gene to a region on chromosome 3 between microsatellite markers RM14360 and RM1332, where the rice orthologue (OsMRP5) of the maize lpa1 gene is located. Sequence analysis of the OsMRP5 gene revealed a single base pair change (C/G-T/A transition) in the sixth exon of XS-lpa2-1 and a 5-bp deletion in the first exon of XS-lpa2-2. OsMRP5 is expressed in both vegetative tissues and developing seeds, and the two mutations do not change the level of RNA transcription. A T-DNA insertion line, 4A-02500, in which OsMRP5 was disrupted, also showed the same high inorganic phosphorus phenotype as Os-lpa-XS110-3 and appeared to be homozygous lethal. PA is significantly reduced in Os-lpa-XS110-2 (similar to 20%) and in 4A-02500 (similar to 90%) seeds compared with their wild type lines, and no PA was detected in Os-lpa-XS110-3 using HPLC analysis. This evidence indicates that the OsMRP5 gene plays an important role in PA metabolism in rice seeds.
- Keywords
- ORYZA-SATIVA L.; INOSITOL PHOSPHATE KINASE; LOW-PHYTATE; MAIZE; BARLEY; EXPRESSION; GENERATION; PHENOTYPE; MUTANTS; ENCODES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28699
- DOI
- 10.1007/S00122-009-1
- ISSN
- 0040-5752
- Article Type
- Article
- Citation
- THEORETICAL AND APPLIED GENETICS, vol. 119, no. 1, page. 75 - 83, 2009-06
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