DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, XH | - |
dc.contributor.author | Zhao, HJ | - |
dc.contributor.author | Liu, QL | - |
dc.contributor.author | Frank, T | - |
dc.contributor.author | Engel, KH | - |
dc.contributor.author | An, GH | - |
dc.contributor.author | Shu, QY | - |
dc.date.accessioned | 2016-04-01T08:45:27Z | - |
dc.date.available | 2016-04-01T08:45:27Z | - |
dc.date.created | 2009-08-17 | - |
dc.date.issued | 2009-06 | - |
dc.identifier.issn | 0040-5752 | - |
dc.identifier.other | 2009-OAK-0000017327 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28699 | - |
dc.description.abstract | Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) is important to the nutritional quality of cereal and legume seeds. PA and its salts with micronutrient cations, such as iron and zinc, cannot be digested by humans and non-ruminant animals, and hence may affect food/feed nutritional value and cause P pollution of groundwater from animal waste. We previously developed a set of low phytic acid (LPA) rice mutant lines with the aim of increasing the nutritional quality of rice. Two of these lines, Os-lpa-XS110-2 (homozygous non-lethal) Os-lpa-XS110-3 (homozygous lethal), contain two mutant alleles of a LPA gene (hereafter XS-lpa2-1 and XS-lpa2-2, respectively). In this study, we mapped the XS-lpa2-1 gene to a region on chromosome 3 between microsatellite markers RM14360 and RM1332, where the rice orthologue (OsMRP5) of the maize lpa1 gene is located. Sequence analysis of the OsMRP5 gene revealed a single base pair change (C/G-T/A transition) in the sixth exon of XS-lpa2-1 and a 5-bp deletion in the first exon of XS-lpa2-2. OsMRP5 is expressed in both vegetative tissues and developing seeds, and the two mutations do not change the level of RNA transcription. A T-DNA insertion line, 4A-02500, in which OsMRP5 was disrupted, also showed the same high inorganic phosphorus phenotype as Os-lpa-XS110-3 and appeared to be homozygous lethal. PA is significantly reduced in Os-lpa-XS110-2 (similar to 20%) and in 4A-02500 (similar to 90%) seeds compared with their wild type lines, and no PA was detected in Os-lpa-XS110-3 using HPLC analysis. This evidence indicates that the OsMRP5 gene plays an important role in PA metabolism in rice seeds. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | THEORETICAL AND APPLIED GENETICS | - |
dc.subject | ORYZA-SATIVA L. | - |
dc.subject | INOSITOL PHOSPHATE KINASE | - |
dc.subject | LOW-PHYTATE | - |
dc.subject | MAIZE | - |
dc.subject | BARLEY | - |
dc.subject | EXPRESSION | - |
dc.subject | GENERATION | - |
dc.subject | PHENOTYPE | - |
dc.subject | MUTANTS | - |
dc.subject | ENCODES | - |
dc.title | Mutations of the multi-drug resistance-associated protein ABC transporter gene 5 result in reduction of phytic acid in rice seeds | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1007/S00122-009-1 | - |
dc.author.google | Xu, XH | - |
dc.author.google | Zhao, HJ | - |
dc.author.google | Liu, QL | - |
dc.author.google | Frank, T | - |
dc.author.google | Engel, KH | - |
dc.author.google | An, GH | - |
dc.author.google | Shu, QY | - |
dc.relation.volume | 119 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 75 | - |
dc.relation.lastpage | 83 | - |
dc.contributor.id | 10184525 | - |
dc.relation.journal | THEORETICAL AND APPLIED GENETICS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | THEORETICAL AND APPLIED GENETICS, v.119, no.1, pp.75 - 83 | - |
dc.identifier.wosid | 000266666500008 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 83 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 75 | - |
dc.citation.title | THEORETICAL AND APPLIED GENETICS | - |
dc.citation.volume | 119 | - |
dc.contributor.affiliatedAuthor | An, GH | - |
dc.identifier.scopusid | 2-s2.0-67349093093 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 37 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ORYZA-SATIVA L. | - |
dc.subject.keywordPlus | INOSITOL PHOSPHATE KINASE | - |
dc.subject.keywordPlus | LOW-PHYTATE | - |
dc.subject.keywordPlus | MAIZE | - |
dc.subject.keywordPlus | BARLEY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | PHENOTYPE | - |
dc.subject.keywordPlus | MUTANTS | - |
dc.subject.keywordPlus | ENCODES | - |
dc.relation.journalWebOfScienceCategory | Agronomy | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Horticulture | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
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