Open Access System for Information Sharing

Login Library

 

Article
Cited 34 time in webofscience Cited 32 time in scopus
Metadata Downloads

Enhanced delivery efficiency of recombinant adenovirus into tumor and mesenchymal stem cells by a novel PTD SCIE SCOPUS

Title
Enhanced delivery efficiency of recombinant adenovirus into tumor and mesenchymal stem cells by a novel PTD
Authors
Youn, JIPark, SHJin, HTLee, CGSeo, SHSong, MYLee, CWSung, YC
Date Issued
2008-11
Publisher
NATURE PUBLISHING GROUP
Abstract
Protein transduction domains (PTDs) are small peptides that facilitate the transduction of large molecules such as polyproteins, DNA and viruses into a eukaryotic cell. Here, we demonstrated that a novel PTD (HP4) derived from herring protamine appeared to enter C6Bu1 rat glioma cell lines more rapidly than other known PTDs such as Tat, Antp and Hph-1. Moreover, HP4 significantly enhanced in vitro transduction of recombinant adenoviruses (rAds) into various cancer cell lines, mesenchymal stem cells (MSCs) and dendritic cells, which are relatively resistant to rAd infection. Enhancement of rAd delivery into C6Bu1 and MSCs by HP4 is 20 and 7 times higher than that by Tat, respectively. The increase in the expression of rAd encoding IL-12N220L by HP4 is proportional to its antitumor effect in the ex vivo transduced mouse colon cancer model. Thus, these results suggest that HP4 could be utilized to improve the transduction efficiency of rAd, resulting in enhanced efficacy of rAd-mediated gene therapy, especially for ex vivo-transduced cell therapy.
Keywords
protein transduction domains; HP4; recombinant adenovirus; INTRACELLULAR PROTEIN DELIVERY; ARGININE-RICH PEPTIDES; MEDIATED GENE-TRANSFER; IN-VIVO; CELLULAR UPTAKE; ANTENNAPEDIA HOMEODOMAIN; TRANSDUCTION DOMAINS; PERMEABLE PEPTIDES; 3RD HELIX; MEMBRANE
URI
https://oasis.postech.ac.kr/handle/2014.oak/22469
DOI
10.1038/CGT.2008.45
ISSN
0929-1903
Article Type
Article
Citation
CANCER GENE THERAPY, vol. 15, no. 11, page. 703 - 712, 2008-11
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

성영철SUNG, YOUNG CHUL
Dept of Life Sciences
Read more

Views & Downloads

Browse