CYTOSOLIC CALCIUM IS ESSENTIAL IN TBE BASAL EXPRESSION OF TYROSINE-HYDROXYLASE GENE
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SCOPUS
- Title
- CYTOSOLIC CALCIUM IS ESSENTIAL IN TBE BASAL EXPRESSION OF TYROSINE-HYDROXYLASE GENE
- Authors
- CHAE, HD; KIM, KT
- Date Issued
- 1995-01-17
- Publisher
- ACADEMIC PRESS INC JNL-COMP SUBSCRIPT
- Abstract
- To test the role of cytosolic calcium in the basal expression of a neuronal gene, promoter activity of the rat tyrosine hydroxylase (TH) gene was monitored upon reduced resting level of intracellular calcium. TH promoter activity was decreased by cell-permeable calcium chelator, BAPTA/AM, in SK-N-BE(2)C human neuroblastoma cells. The cAMP response element (CRE) was mapped to the calcium responsible element by mutational and deletional analysis of the 5' upstream promoter region. Gel shift assay showed 2 CRE-specific DNA-protein complexes. The quantities of specific complexes were markedly decreased in BAPTA/AM-treated cells. These data suggest that resting level of intracellular calcium has a critical role in the basal expression of TH gene through the regulation of the binding of nuclear proteins to the CRE motif in the promoter. (C) 1995 Academic Press, Inc.
- Keywords
- CELL-SPECIFIC EXPRESSION; NERVE GROWTH-FACTOR; MEMBRANE DEPOLARIZATION; CHLORAMPHENICOL ACETYLTRANSFERASE; REGULATORY ELEMENTS; RESPONSIVE ELEMENT; CYCLIC-AMP; PC12 CELLS; TRANSCRIPTION; PHOSPHORYLATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/21863
- DOI
- 10.1006/bbrc.1995.1093
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 206, no. 2, page. 659 - 666, 1995-01-17
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