Induction of apoptosis in p53-deficient human hepatoma cell line by wild-type p53 gene transduction: Inhibition by antioxidant
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- Title
- Induction of apoptosis in p53-deficient human hepatoma cell line by wild-type p53 gene transduction: Inhibition by antioxidant
- Authors
- Lee, KH; Kim, KC; Jung, YJ; Ham, YH; Jang, JJ; Kwon, H; Sung, YC; Kim, SH; Han, SK; Kim, CM
- Date Issued
- 2001-08-31
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Abstract
- We investigated the role of wild-type (wt)-p53 as an inducer of apoptotic cell death in human hepatoma cell lines. Following the retrovirus-mediated transduction of the wt-p53 gene, Hep3B cells lacking the endogenous p53 expression began to die through apoptosis in 4 h. They showed a maximal apoptotic death at 12 h, whereas HepG2 cells expressing endogenous p53 did not. However, the transduction of the wt-p53 gene elicited growth suppression of both Hep3B and HepG2 cells. P21(WAF1/CIP1), a p53-inducible cell cycle inhibitor, was induced, not only in Hep3B cells undergoing apoptosis, but also in HepG2 cells. The kinetics of the p21(WAF1/CIP1) induction, DNA fragmentation, and growth suppression of the Hep3B cells showed that DNA fragmentation and growth suppression progressed rapidly following p21(WAF1/CIP1) accumulation. N-acetylcysteine or glutathione, potent antioxidants, strongly inhibited the DNA fragmentation, but did not reduce the elevated level of p21(WAF1/CIP1). These findings suggested that p21(WAF1/CIP1) was not a critical mediator for the execution of p53-mediated apoptosis, although it contributed to the growth inhibition of cells undergoing apoptosis. Furthermore, p53-mediated apoptosis could be repressed by antioxidants.
- Keywords
- p21(WAF1-CIP1); p53; antioxidant; apoptosis; hepatocellular carcinoma; HUMAN HEPATOCELLULAR CARCINOMAS; LUNG-CANCER CELLS; GROWTH SUPPRESSION; ADENOVIRUS; EXPRESSION; PROTEIN; WAF1/CIP1; RADICALS; THERAPY; ARREST
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19415
- ISSN
- 1016-8478
- Article Type
- Article
- Citation
- MOLECULES AND CELLS, vol. 12, no. 1, page. 17 - 24, 2001-08-31
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