Deacetylated alpha beta-tubulin acts as a positive regulator of Rheb GTPase through increasing its GTP-loading
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- Title
- Deacetylated alpha beta-tubulin acts as a positive regulator of Rheb GTPase through increasing its GTP-loading
- Authors
- Lee, MN; Koh, A; Park, D; Jang, JH; Kwak, D; Jeon, H; Kim, J; Choi, EJ; Jeong, H; Suh, PG; Ryu, SH
- Date Issued
- 2013-02
- Publisher
- ELSEVIER SCIENCE INC
- Abstract
- Ras homolog enriched in brain (Rheb) regulates diverse cellular functions by modulating its nucleotide-bound status. Although Rheb contains a high basal GTP level, the regulatory mechanism of Rheb is not well understood. In this study, we propose soluble alpha beta-tubulin acts as a constitutively active Rheb activator, which may explain the reason why Rheb has a high basal GTP levels. We found that soluble alpha beta-tubulin is a direct Rheb-binding protein and that its deacetylated form has a high binding affinity for Rheb. Modulation of both soluble and acetylated alpha beta-tubulin levels affects the level of GTP-bound Rheb. This occurs in the mitotic phase in which the level of acetylated alpha beta-tubulin is increased but that of GTP-bound Rheb is decreased. Constitutively active Rheb-overexpressing cells showed an abnormal mitotic progression, suggesting the deacetylated alpha beta-tubulin-mediated regulation of Rheb status may be important for proper mitotic progression. Taken together, we propose that deacetylated soluble alpha beta-tubulin is a novel type of positive regulator of Rheb and may play a role as a temporal regulator for Rheb during the cell cycle. (C) 2012 Elsevier Inc. All rights reserved.
- Keywords
- Rheb; Soluble tubulin; mTOR; Mitosis; CELL-CYCLE PROGRESSION; RIBOSOME ENTRY SITE; MICROTUBULE DYNAMICS; SIGNAL-TRANSDUCTION; MAMMALIAN TARGET; IN-VIVO; GROWTH; PROTEIN; ACTIVATION; TSC2
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16192
- DOI
- 10.1016/J.CELLSIG.2012.11.006
- ISSN
- 0898-6568
- Article Type
- Article
- Citation
- CELLULAR SIGNALLING, vol. 25, no. 2, page. 539 - 551, 2013-02
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