Wedelolactone inhibits adipogenesis through the ERK pathway in human adipose tissue-derived mesenchymal stem cells
SCIE
SCOPUS
- Title
- Wedelolactone inhibits adipogenesis through the ERK pathway in human adipose tissue-derived mesenchymal stem cells
- Authors
- Lim, S; Jang, HJ; Park, EH; Kim, JK; Kim, JM; Kim, EK; Yea, K; Kim, YH; Lee-Kwon, W; Ryu, SH; Suh, PG
- Date Issued
- 2012-11
- Publisher
- JOURNAL OF CELLULAR BIOCHEMISTRY
- Abstract
- Wedelolactone is an herbal medicine that is used to treat septic shock, hepatitis and venom poisoning. Although in differentiated and cancer cells, wedelolactone has been identified as anti-inflammatory, growth inhibitory, and pro-apoptotic, the effects of wedelolactone on stem cell differentiation remain largely unknown. Here, we report that wedelolactone inhibits the adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAMSCs). Wedelolactone reduced the formation of lipid droplets and the expression of adipogenesis-related proteins, such as CCAAT enhancer-binding protein-a (C/EBP-a), peroxisome proliferator-activated receptor-? (PPAR-?), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein aP2 (aP2). Wedelolactone mediated this process by sustaining ERK activity. In addition, inhibition of ERK activity with PD98059 resulted in reversion of the wedelolactone-mediated inhibition of adipogenic differentiation. Taken together, these results indicate that wedelolactone inhibits adipogenic differentiation through ERK pathway and suggest a novel inhibitory effect of wedelolactone on adipogenic differentiation in hAMSCs. J. Cell. Biochem. 113: 34363445, 2012. (C) 2012 Wiley Periodicals, Inc.
- Keywords
- ADIPOGENESIS; MESENCHYMAL STEM CELLS; WEDELOLACTONE; ACTIVATED PROTEIN-KINASE; MARROW STROMAL CELLS; 3T3-L1 ADIPOCYTE DIFFERENTIATION; N-TERMINAL KINASE; ANDROGEN RECEPTOR; PROSTATE-CANCER; GAMMA PATHWAY; PPAR-GAMMA; PHOSPHORYLATION; PROLIFERATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16185
- DOI
- 10.1002/JCB.24220
- ISSN
- 0730-2312
- Article Type
- Article
- Citation
- JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 113, no. 11, page. 3436 - 3445, 2012-11
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