Efficacy of rhBMP-2 loaded PCL/PLGA/beta-TCP guided bone regeneration membrane fabricated by 3D printing technology for reconstruction of calvaria defects in rabbit
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SCOPUS
- Title
- Efficacy of rhBMP-2 loaded PCL/PLGA/beta-TCP guided bone regeneration membrane fabricated by 3D printing technology for reconstruction of calvaria defects in rabbit
- Authors
- Shim, JH; Yoon, MC; Jeong, CM; Jang, J; Jeong, SI; Cho, DW; Huh, JB
- Date Issued
- 2014-12
- Publisher
- IOP PUBLISHING LTD
- Abstract
- We successfully fabricated a three-dimensional (3D) printing-based PCL/PLGA/beta-TCP guided bone regeneration (GBR) membrane that slowly released rhBMP-2. To impregnate the GBR membrane with intact rhBMP-2, collagen solution encapsulating rhBMP-2 (5 mu g ml(-1)) was infused into pores of a PCL/PLGA/beta-TCP membrane constructed using a 3D printing system with four dispensing heads. In a release profile test, sustained release of rhBMP-2 was observed for up to 28 d. To investigate the efficacy of the GBR membrane on bone regeneration, PCL/PLGA/beta-TCP membranes with or without rhBMP-2 were implanted in an 8 mm calvaria defect of rabbits. Bone formation was evaluated at weeks 4 and 8 histologically and histomorphometrically. A space making ability of the GBR membrane was successfully maintained in both groups, and significantly more new bone was formed at post-implantation weeks 4 and 8 by rhBMP-2 loaded GBR membranes. Interestingly, implantation with rhBMP-2 loaded GBR membranes led to almost entire healing of calvaria defects within 8 weeks.
- Keywords
- guided bone regeneration membrane; 3D printing; bone morphogenetic proteins-2 (BMP-2); calvaria defect; MORPHOGENETIC PROTEIN-2; PERI-IMPLANT; SOFT-TISSUE; PORE-SIZE; IN-VIVO; SCAFFOLD; BMP-2; OSTEOGENESIS; PRINCIPLE; DELIVERY
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/14250
- DOI
- 10.1088/1748-6041/9/6/065006
- ISSN
- 1748-6041
- Article Type
- Article
- Citation
- BIOMEDICAL MATERIALS, vol. 9, no. 6, 2014-12
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