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Cited 64 time in webofscience Cited 66 time in scopus
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Ultrarapid Detection of Pathogenic Bacteria Using a 3D Immunomagnetic Flow Assay

Title
Ultrarapid Detection of Pathogenic Bacteria Using a 3D Immunomagnetic Flow Assay
Authors
Lee, WKwon, DChung, BJung, GYAu, AFolch, AJeon, S
POSTECH Authors
Jung, GYJeon, S
Date Issued
May-2014
Publisher
AMER CHEMICAL SOC
Abstract
We developed a novel 3D immunomagnetic flow assay for the rapid detection of pathogenic bacteria in a large-volume food sample. Antibody-functionalized magnetic nanoparticle clusters (AbMNCs) were magnetically immobilized on the surfaces of a 3D-printed cylindrical microchannel. The injection of a Salmonella-spiked sample solution into the microchannel produced instant binding between the AbMNCs and the Salmonella bacteria due to their efficient collisions. Nearly perfect capture of the AbMNCs and AbMNCs-Salmonella complexes was achieved under a high flow rate by stacking permanent magnets with spacers inside the cylindrical separator to maximize the magnetic force. The concentration of the bacteria in solution was determined using ATP luminescence measurements. The detection limit was better than 10 cfu/mL, and the overall assay time, including the binding, rinsing, and detection steps for a 10 mL sample took less than 3 min. To our knowledge, the 3D immunomagnetic flow assay described here provides the fastest high-sensitivity, high-capacity method for the detection of pathogenic bacteria.
Keywords
MULTIPLE PATHOGENS; SEPARATION; MICROFLUIDICS
URI
http://oasis.postech.ac.kr/handle/2014.oak/13912
DOI
10.1021/AC501436D
ISSN
0003-2700
Article Type
Article
Citation
ANALYTICAL CHEMISTRY, vol. 86, no. 13, page. 6683 - 6688, 2014-05
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정규열JUNG, GYOO YEOL
Dept. of Chemical Enginrg
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