Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing
SCIE
SCOPUS
- Title
- Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing
- Authors
- Choi, TY; Denton, ML; Noojin, GD; Estlack, LE; Shrestha, R; Rockwell, BA; Thomas, R; Kim, D
- Date Issued
- 2014-09
- Publisher
- SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
- Abstract
- A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-mu m beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipette-based thermal sensors of size ranging from 2 to 4 mu m into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73 degrees C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/10682
- DOI
- 10.1117/1.JBO.19.9.097003
- ISSN
- 1083-3668
- Article Type
- Article
- Citation
- JOURNAL OF BIOMEDICAL OPTICS, vol. 19, no. 9, 2014-09
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