Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
SCIE
SCOPUS
- Title
- Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
- Authors
- LEE, DONGHYEON; PARK, SEHAN; LEE, KEONDO; KIM, JANGWOO; PARK, GISU; NAM, KIHYUN; BAEK, SANGWON; CHUNG, WAN KYUN; LEE, JONG LAM; CHO, YUNJE; PARK, JAEHYUN
- Date Issued
- 2020-04
- Publisher
- Blackwell Publishing Inc.
- Abstract
- Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/103443
- DOI
- 10.1107/S1600576720002423
- ISSN
- 0021-8898
- Article Type
- Article
- Citation
- Journal of Applied Crystallography, vol. 53, page. 477 - 485, 2020-04
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- There are no files associated with this item.
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