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Cited 4 time in webofscience Cited 5 time in scopus
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Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography

Title
Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography
Authors
LEE, DONGHYEONPARK, SEHANLEE, KEONDOKIM, JANGWOOPARK, GISUNAM, KIHYUNBAEK, SANGWONCHUNG, WAN KYUNLEE, JONG LAMCHO, YUNJEPARK, JAEHYUN
Date Issued
2020-04
Publisher
Blackwell Publishing Inc.
Abstract
Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution.
URI
https://oasis.postech.ac.kr/handle/2014.oak/103443
ISSN
0021-8898
Article Type
Article
Citation
Journal of Applied Crystallography, vol. 53, page. 477 - 485, 2020-04
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이종람LEE, JONG LAM
Dept of Materials Science & Enginrg
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