DC Field | Value | Language |
---|---|---|
dc.contributor.author | LEE, DONGHYEON | - |
dc.contributor.author | PARK, SEHAN | - |
dc.contributor.author | LEE, KEONDO | - |
dc.contributor.author | KIM, JANGWOO | - |
dc.contributor.author | PARK, GISU | - |
dc.contributor.author | NAM, KIHYUN | - |
dc.contributor.author | BAEK, SANGWON | - |
dc.contributor.author | CHUNG, WAN KYUN | - |
dc.contributor.author | LEE, JONG LAM | - |
dc.contributor.author | CHO, YUNJE | - |
dc.contributor.author | PARK, JAEHYUN | - |
dc.date.accessioned | 2020-04-16T01:50:05Z | - |
dc.date.available | 2020-04-16T01:50:05Z | - |
dc.date.created | 2020-04-14 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 0021-8898 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/103443 | - |
dc.description.abstract | Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution. | - |
dc.language | English | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.relation.isPartOf | Journal of Applied Crystallography | - |
dc.title | Application of a high-throughput microcrystal delivery system to serial femtosecond crystallography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1107/S1600576720002423 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Journal of Applied Crystallography, v.53, pp.477 - 485 | - |
dc.identifier.wosid | 000524119500018 | - |
dc.citation.endPage | 485 | - |
dc.citation.startPage | 477 | - |
dc.citation.title | Journal of Applied Crystallography | - |
dc.citation.volume | 53 | - |
dc.contributor.affiliatedAuthor | LEE, DONGHYEON | - |
dc.contributor.affiliatedAuthor | LEE, KEONDO | - |
dc.contributor.affiliatedAuthor | KIM, JANGWOO | - |
dc.contributor.affiliatedAuthor | BAEK, SANGWON | - |
dc.contributor.affiliatedAuthor | CHUNG, WAN KYUN | - |
dc.contributor.affiliatedAuthor | LEE, JONG LAM | - |
dc.contributor.affiliatedAuthor | CHO, YUNJE | - |
dc.contributor.affiliatedAuthor | PARK, JAEHYUN | - |
dc.identifier.scopusid | 2-s2.0-85083072915 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | FREE-ELECTRON LASER | - |
dc.subject.keywordPlus | DIFFRACTION | - |
dc.subject.keywordAuthor | high-throughput microcrystal delivery | - |
dc.subject.keywordAuthor | HT-MCD | - |
dc.subject.keywordAuthor | microcrystal delivery systems | - |
dc.subject.keywordAuthor | serial femtosecond crystallography | - |
dc.subject.keywordAuthor | X-ray free-electron lasers | - |
dc.subject.keywordAuthor | XFELs | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Crystallography | - |
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