Single-molecule, real-time measurement of enzyme kinetics by alternating-laser excitation fluorescence resonance energy transfer
SCIE
SCOPUS
- Title
- Single-molecule, real-time measurement of enzyme kinetics by alternating-laser excitation fluorescence resonance energy transfer
- Authors
- Lee, NK; Hye Ran Koh; Kyu Young Han; Ji Hyun Lee; Seung Keun Kim
- Date Issued
- 2010-06
- Publisher
- The Royal Society of Chemistry
- Abstract
- Using a single-molecule fluorescence method uniquely suitable for binding assay, alternating-laser excitation fluorescence resonance energy transfer (ALEX-FRET), we accurately measured the cleavage rate of 8-17 deoxyribozyme, an RNA-cleaving enzyme, at the single-molecule level in real time with a minimum consumption of samples, i.e., at least three orders of magnitude smaller than used in the conventional ensemble FRET method.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/9968
- DOI
- 10.1039/C002666B
- ISSN
- 1359-7345
- Article Type
- Article
- Citation
- CHEMICAL COMMUNICATIONS, vol. 46, no. 26, page. 4683 - 4685, 2010-06
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