Open Access System for Information Sharing

Login Library

 

Article
Cited 29 time in webofscience Cited 31 time in scopus
Metadata Downloads

Sepsis-Like Systemic Inflammation Induced by Nano-Sized Extracellular Vesicles From Feces SCIE SCOPUS

Title
Sepsis-Like Systemic Inflammation Induced by Nano-Sized Extracellular Vesicles From Feces
Authors
Park, Kyong-SuLee, JaewookLee, ChangjinPark, Hyun TaekKim, Jung-WookKim, Oh YounKim, Sae RomRadinger, MadeleineJung, Hoe-YunePark, JaesungLotvall, JanGho, Yong Song
Date Issued
2018-08
Publisher
Frontiers Media S.A.
Abstract
Nano-sized extracellular vesicles (EVs), including exosomes, microvesicles, and other types of vesicles, are released by most mammalian cells and bacteria. We here ask whether feces contain EVs of mammalian and/or bacterial origin, and whether these EVs induce systemic inflammation. Fecal extracellular vesicles (fEVs) were isolated from mice and humans. The presence of EVs from Gram-negative and Gram-positive bacteria was detected by enzyme-linked immunosorbent assay using anti-lipid A and anti-lipoteichoic acid antibodies, whereas Western blot using anti-beta-actin antibody was employed to detect host-derived EVs in the fEVs. Further, fEVs were administered into mice by intraperitoneal injection, and inflammatory responses were investigated in the peritoneum, blood, and lungs. The role of TLR2 and TLR4 were studied using knockout mice. Significant quantities of EVs were present in feces from mice as well as humans, and derived from Gram-negative and Gram-positive bacteria, as well as the host. Bacteria-free fEVs introduced into the peritoneum induced local and systemic inflammation (including in the lungs), but fEVs from germ-free animals had weaker effects. This pronounced local and systemic inflammatory responses seemed to be induced by EVs from both Gram-negative and Gram-positive bacteria, and was attenuated in mice lacking TLR2 or TLR4. Our findings show that fEVs cause sepsis-like systemic inflammation, when introduced intraperitoneally, a process regulated by TLR2 and TLR4.
URI
https://oasis.postech.ac.kr/handle/2014.oak/99266
DOI
10.3389/fmicb.2018.01735
ISSN
1664-302X
Article Type
Article
Citation
Frontiers in Microbiology, vol. 9, no. AUG, 2018-08
Files in This Item:

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

박재성PARK, JAE SUNG
Dept of Mechanical Enginrg
Read more

Views & Downloads

Browse