Open Access System for Information Sharing

Login Library

 

Article
Cited 5 time in webofscience Cited 6 time in scopus
Metadata Downloads

A continuous droplet electroporation system for high throughput processing

Title
A continuous droplet electroporation system for high throughput processing
Authors
Yoo, Byeong SunIm, Do JinAhn, Myung MoPark, Sung JinKim, Yeong HunUm, Tae WoongKang, In Seok
Date Issued
2018-12
Publisher
ROYAL SOC CHEMISTRY
Abstract
A continuous droplet electroporation (EP) system capable of handling a billion cells has been proposed and demonstrated using a proof-of-concept prototype design. Numerical simulations were conducted to design the new system and to compare the continuous droplet EP system with the previous single droplet EP system. Through parametric studies on the applied voltage and flow rate, a much higher cyan fluorescent protein transgene expression efficiency (38.8 +/- 8.9%) was accomplished over that of the previous single droplet EP system. A parallel continuous droplet EP system is also demonstrated by introducing additional electrode pairs into the continuous droplet EP system to achieve ultrahigh throughput. Finally, the significance and meaning of the present work and future development direction have been discussed.
URI
https://oasis.postech.ac.kr/handle/2014.oak/95468
ISSN
0003-2654
Article Type
Article
Citation
ANALYST, vol. 143, no. 23, page. 5785 - 5791, 2018-12
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

강인석KANG, IN SEOK
Dept. of Chemical Enginrg
Read more

Views & Downloads

Browse