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Cited 15 time in webofscience Cited 15 time in scopus
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Two-photon microscopy of Paneth cells in the small intestine of live mice SCIE SCOPUS

Title
Two-photon microscopy of Paneth cells in the small intestine of live mice
Authors
Jang, Won HyukPark, AreumWang, TaejunKim, Chan JohngChang, HoonchulYang, Bo-GieKim, Myoung JoonMyung, Seung-JaeIm, Sin-HyeogJang, Myoung HoKim, You-MeKim, Ki Hean
Date Issued
2018-09
Publisher
NATURE PUBLISHING GROUP
Abstract
Paneth cells are one of the principal epithelial cell types in the small intestine, located at the base of intestinal crypts. Paneth cells play key roles in intestinal host-microbe homeostasis via granule secretion, and their dysfunction is implicated in pathogenesis of several diseases including Crohn's disease. Despite their physiological importance, study of Paneth cells has been hampered by the limited accessibility and lack of labeling methods. In this study, we developed a simple in vivo imaging method of Paneth cells in the intact mouse small intestine by using moxifloxacin and two-photon microscopy (TPM). Moxifloxacin, an FDA-approved antibiotic, was used for labeling cells and its fluorescence was strongly observed in Paneth cell granules by TPM. Moxifloxacin labeling of Paneth cell granules was confirmed by molecular counterstaining. Comparison of Paneth cells in wild type, genetically obese (ob/ob), and germ-free (GF) mice showed different granule distribution. Furthermore, Paneth cell degranulation was observed in vivo. Our study suggests that TPM with moxifloxacin labeling can serve as a useful tool for studying Paneth cell biology and related diseases.
URI
https://oasis.postech.ac.kr/handle/2014.oak/94627
DOI
10.1038/s41598-018-32640-7
ISSN
2045-2322
Article Type
Article
Citation
SCIENTIFIC REPORTS, vol. 8, no. 1, 2018-09
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김기현KIM, KI HEAN
Dept of Mechanical Enginrg
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