Efficient Conversion of Acetate to 3-Hydroxypropionic Acid by Engineered Escherichia coli
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SCOPUS
- Title
- Efficient Conversion of Acetate to 3-Hydroxypropionic Acid by Engineered Escherichia coli
- Authors
- JIHUN, LEE; CHA, SANG HAK; KANG, CHAEWON; LIM, HYUNGYU; Jung, Gyoo Yeol
- Date Issued
- 2018-11
- Publisher
- MDPI
- Abstract
- Acetate, which is an abundant carbon source, is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we initially introduced heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield).
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94486
- DOI
- 10.3390/catal8110525
- ISSN
- 2073-4344
- Article Type
- Article
- Citation
- CATALYSTS, vol. 8, no. 11, 2018-11
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