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Genome-wide maps of chromatin state in pluripotent and lineage-committed cells SCIE SCOPUS

Title
Genome-wide maps of chromatin state in pluripotent and lineage-committed cells
Authors
KIM, TAE KYUNG
Date Issued
2007-08-02
Publisher
NATURE PUBLISHING GROUP
Abstract
We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find that lysine 4 and lysine 27 trimethylation effectively discriminates genes that are expressed, poised for expression, or stably repressed, and therefore reflect cell state and lineage potential. Lysine 36 trimethylation marks primary coding and non-coding transcripts, facilitating gene annotation. Trimethylation of lysine 9 and lysine 20 is detected at satellite, telomeric and active long-terminal repeats, and can spread into proximal unique sequences. Lysine 4 and lysine 9 trimethylation marks imprinting control regions. Finally, we show that chromatin state can be read in an allele-specific manner by using single nucleotide polymorphisms. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cell populations.
URI
https://oasis.postech.ac.kr/handle/2014.oak/94332
DOI
10.1038/nature06008
ISSN
0028-0836
Article Type
Article
Citation
NATURE, vol. 448, no. 7153, page. 553 - 560, 2007-08-02
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김태경KIM, TAE KYUNG
Dept of Life Sciences
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