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Widespread transcription at neuronal activity-regulated enhancers SCIE SCOPUS

Title
Widespread transcription at neuronal activity-regulated enhancers
Authors
KIM, TAE KYUNG
Date Issued
2010-05-13
Publisher
NATURE PUBLISHING GROUP
Abstract
We used genome-wide sequencing methods to study stimulus-dependent enhancer function in mouse cortical neurons. We identified similar to 12,000 neuronal activity-regulated enhancers that are bound by the general transcriptional co-activator CBP in an activity-dependent manner. A function of CBP at enhancers may be to recruit RNA polymerase II (RNAPII), as we also observed activity-regulated RNAPII binding to thousands of enhancers. Notably, RNAPII at enhancers transcribes bi-directionally a novel class of enhancer RNAs (eRNAs) within enhancer domains defined by the presence of histone H3 monomethylated at lysine 4. The level of eRNA expression at neuronal enhancers positively correlates with the level of messenger RNA synthesis at nearby genes, suggesting that eRNA synthesis occurs specifically at enhancers that are actively engaged in promoting mRNA synthesis. These findings reveal that a widespread mechanism of enhancer activation involves RNAPII binding and eRNA synthesis.
URI
https://oasis.postech.ac.kr/handle/2014.oak/94324
DOI
10.1038/nature09033
ISSN
0028-0836
Article Type
Article
Citation
NATURE, vol. 465, no. 7295, page. 182 - 187, 2010-05-13
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김태경KIM, TAE KYUNG
Dept of Life Sciences
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