Conformational Substates of Myoglobin Intermediate Resolved by Picosecond X-ray Solution Scattering
SCIE
SCOPUS
- Title
- Conformational Substates of Myoglobin Intermediate Resolved by Picosecond X-ray Solution Scattering
- Authors
- Key Young Oang; Jong Goo Kim; Cheolhee Yang; Tae Wu Kim; Youngmin Kim; KIM, KYUNG HWAN; Jeongho Kim; Hyotcherl Ihee
- Date Issued
- 2014-03-06
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Conformational substates of proteins are generally considered to play important roles in regulating protein functions, but an understanding of how they influence the structural dynamics and functions of the proteins has been elusive. Here, we investigate the structural dynamics of sperm whale myoglobin associated with the conformational substates using picosecond X-ray solution scattering. By applying kinetic analysis considering all of the plausible candidate models, we establish a kinetic model for the entire cycle of the protein transition in a wide time range from 100 ps to 10 ms. Four structurally distinct intermediates are formed during the cycle, and most importantly, the transition from the first intermediate to the second one (B -> C) occurs biphasically. We attribute the biphasic kinetics to the involvement of two conformational substates of the first intermediate, which are generated by the interplay between the distal histidine and the photodissociated CO.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94142
- DOI
- 10.1021/jz4027425
- ISSN
- 1948-7185
- Article Type
- Article
- Citation
- Journal of Physical Chemistry Letters, vol. 5, no. 5, page. 804 - 808, 2014-03-06
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.