High throughput genetic screening of higher crossover rate mutants in
Arabidopsis
- Title
- High throughput genetic screening of higher crossover rate mutants in
Arabidopsis
- Authors
- CHOI, KYUHA; KIM, JUHYUN; KIM, JAEIL; PARK, JI HYE; KIM, HANUL
- Date Issued
- 2018-06-12
- Publisher
- Gordon Research Conference
- Abstract
- Meiotic homologous recombination generates crossovers from programmed DSBs via
interhomolog repair pathways. The meiotic crossover frequency and distribution affect genetic
diversity in population and breeding. Crossover number is restricted to ~1-3 along a
chromosome by anti-crossover factors including FANCM, RECQ4 and FIGL1 in Arabidopsis.
However, the mechanism how the excessive DSBs (~40-50 per chromosome) are repaired to
only 1-3 crossovers remains further elucidated. We performed a high throughput genetic
screening of higher crossover rate (hcr) mutants by using fluorescent seed-based system,
enabling the measurement of crossover frequency in individual plants from EMS-driven mutant
pool. We have isolated three hcr mutants (hcr1, hcr2, hcr3) that were confirmed to be new anticrossover
mutants by genetic analysis and deep-sequencing. In this poster we will present
characterization of hcr2 and hcr3 mutants, including mapping of them and their effects on
crossover frequency.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94049
- Article Type
- Conference
- Citation
- 14th Meiosis Gordon Research Conference, page. 79 - 79, 2018-06-12
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.