SIRP alpha/CD172a Regulates Eosinophil Homeostasis
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- Title
- SIRP alpha/CD172a Regulates Eosinophil Homeostasis
- Authors
- Garcia, NV; Umemoto, E; Saito, Y; Yamasaki, M; Hata, E; Matozaki, T; Murakami, M; Jung, YJ; Woo, SY; Seoh, JY; Jang, MH; Aozasa, K; Miyasaka, M
- Date Issued
- 2011-09-01
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Abstract
- Eosinophils are abundant in the lamina propria of the small intestine, but they rarely show degranulation in situ under steady-state conditions. In this study, using two novel mAbs, we found that intestinal eosinophils constitutively expressed a high level of an inhibitory receptor signal regulatory protein alpha (SIRP alpha)/CD172a and a low, but significant, level of a tetraspanin CD63, whose upregulation is closely associated with degranulation. Cross-linking SIRP alpha/CD172a on the surface of wild-type eosinophils significantly inhibited the release of eosinophil peroxidase induced by the calcium ionophore A23187, whereas this cross-linking effect was not observed in eosinophils isolated from mice expressing a mutated SIRP alpha/CD172a that lacks most of its cytoplasmic domain (SIRP alpha Cyto(-/-)). The SIRP alpha Cyto(-/-) eosinophils showed reduced viability, increased CD63 expression, and increased eosinophil peroxidase release with or without A23187 stimulation in vitro. In addition, SIRP alpha Cyto(-/-) mice showed increased frequencies of Annexin V-binding eosinophils and free MBP(+)CD63(+) extracellular granules, as well as increased tissue remodeling in the small intestine under steady-state conditions. Mice deficient in CD47, which is a ligand for SIRP alpha/CD172a, recapitulated these phenomena. Moreover, during Th2-biased inflammation, increased eosinophil cell death and degranulation were obvious in a number of tissues, including the small intestine, in the SIRP alpha Cyto(-/-) mice compared with wild-type mice. Collectively, our results indicated that SIRP alpha/CD172a regulates eosinophil homeostasis, probably by interacting with CD47, with substantial effects on eosinophil survival. Thus, SIRP alpha/CD172a is a potential therapeutic target for eosinophil-associated diseases. The Journal of Immunology, 2011, 187: 2268-2277.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/92455
- DOI
- 10.4049/JIMMUNOL.1101008
- ISSN
- 0022-1767
- Article Type
- Article
- Citation
- JOURNAL OF IMMUNOLOGY, vol. 187, no. 5, page. 2268 - 2277, 2011-09-01
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