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Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography

Title
Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography
Authors
Kwon, DLee, SAhn, MMKang, ISPark, KHJeon, S
POSTECH Authors
Kang, ISJeon, S
Date Issued
Jul-2015
Publisher
ELSEVIER SCIENCE BV
Abstract
A colorimetric method that uses platinum-coated magnetic nanoparticle clusters (Pt/MNCs) and magnetophoretic chromatography is developed to detect pathogenic bacteria. Half-fragments of monoclonal Escherichia coli O157:H7 (EC) antibodies were functionalized to Pt/MNCs and used to capture Escherichia coli bacteria in milk. After magnetic separation of free Pt/MNCs and Pt/MNC-EC complexes from the milk, a precision pipette was used to imbibe the Escherichia coli-containing solution, then a viscous polyethylene glycol solution. Due to difference in viscosities, the solutions separate into two liquid layers inside the pipette tip. The Pt/MNC-EC complexes were separated from the free Pt/MNCs by applying an external magnetic field, then added to a tetramethylbenzidine (TMB) solution. Catalytic oxidation of TMB by Pt produced color changes of the solution, which enabled identification of the presence of 10 cfu mL(-1) Escherichia coli bacteria with the naked eye. The total assay time including separation, binding and detection was 30 min. (C) 2015 Elsevier B.V. All rights reserved.
A colorimetric method that uses platinum-coated magnetic nanoparticle clusters (Pt/MNCs) and magnetophoretic chromatography is developed to detect pathogenic bacteria. Half-fragments of monoclonal Escherichia coli O157:H7 (EC) antibodies were functionalized to Pt/MNCs and used to capture Escherichia coli bacteria in milk. After magnetic separation of free Pt/MNCs and Pt/MNC-EC complexes from the milk, a precision pipette was used to imbibe the Escherichia coli-containing solution, then a viscous polyethylene glycol solution. Due to difference in viscosities, the solutions separate into two liquid layers inside the pipette tip. The Pt/MNC-EC complexes were separated from the free Pt/MNCs by applying an external magnetic field, then added to a tetramethylbenzidine (TMB) solution. Catalytic oxidation of TMB by Pt produced color changes of the solution, which enabled identification of the presence of 10 cfu mL(-1) Escherichia coli bacteria with the naked eye. The total assay time including separation, binding and detection was 30 min. (C) 2015 Elsevier B.V. All rights reserved.
URI
http://oasis.postech.ac.kr/handle/2014.oak/92373
DOI
10.1016/J.ACA.2015.04.044
ISSN
0003-2670
Article Type
Article
Citation
ANALYTICA CHIMICA ACTA, vol. 883, page. 61 - 66, 2015-07
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강인석KANG, IN SEOK
Dept. of Chemical Enginrg
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