Open Access System for Information Sharing

Login Library

 

Article
Cited 2 time in webofscience Cited 2 time in scopus
Metadata Downloads

Cell-Engineered Nanovesicle as a Surrogate Inducer of Contact-Dependent Stimuli

Title
Cell-Engineered Nanovesicle as a Surrogate Inducer of Contact-Dependent Stimuli
Authors
Kim, JunhoHan, ChugminJo, WonjuKang, SehongCho, SiwooJeong, DayeongGho, Yong SongPark, Jaesung
Date Issued
Sep-2017
Publisher
WILEY
Abstract
Heterotypic interactions between cells are crucial in various biological phenomena. Particularly, stimuli that regulate embryonic stem cell (ESC) fate are often provided from neighboring cells. However, except for feeder cultures, no practical methods are identified that can provide ESCs with contact-dependent cell stimuli. To induce contact-dependent cell stimuli in the absence of living cells, a novel method that utilizes cell-engineered nano-vesicles (CNVs) that are made by extruding living cells through microporous membranes is described. Protein compositions of CNVs are similar to their originating cells, as well as freely diffusible and precisely scalable. Treatment of CNVs produced from three different stromal cells successfully induces the same effect as feeder cultures. The results suggest that the effects of CNVs are mainly mediated by membrane-associated components. The use of CNVs might constitute a novel and efficient tool for ESC research.
URI
http://oasis.postech.ac.kr/handle/2014.oak/50546
ISSN
2192-2640
Article Type
Article
Citation
ADVANCED HEALTHCARE MATERIALS, vol. 6, no. 17, 2017-09
Files in This Item:

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

박재성PARK, JAE SUNG
Dept of Mechanical Enginrg
Read more

Views & Downloads

Browse