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Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation SCIE SCOPUS

Title
Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation
Authors
Song, Kwang HoonLee, JaehyunJung, Hong-RyulPark, HyoungJunDoh, Junsang
Date Issued
2017-09
Publisher
Nature Publishing Group
Abstract
T cells navigate diverse microenvironments to perform immune responses. Micro-scale topographical structures within the tissues, which may inherently exist in normal tissues or may be formed by inflammation or injury, can influence T cell migration, but how T cell migration is affected by such topographical structures have not been investigated. In this study, we fabricated ramp-like structures with a 5 mu m height and various slopes, and observed T cells climbing up the ramp-like structures. T cells encountering the ramp-like structures exhibited MLC accumulation near head-tail junctions contacting the ramp-like structures, and made turns to the direction perpendicular to the ramp-like structures. Pharmacological study revealed that lamellipodia formation mediated by arp2/3 and contractility regulated by myosin light chain kinase (MLCK) were responsible for the intriguing turning behavior of T cells climbing the ramp-like structures. Arp2/3 or MLCK inhibition substantially reduced probability of T cells climbing sharp-edged ramp-like structures, indicating intriguing turning behavior of T cells mediated by lamellipodia formation and MLCK activity may be important for T cells to access inflamed or injured tissues with abrupt topographical changes.
URI
https://oasis.postech.ac.kr/handle/2014.oak/50399
DOI
10.1038/s41598-017-11938-y
ISSN
2045-2322
Article Type
Article
Citation
Scientific Reports, vol. 7, 2017-09
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도준상DOH, JUN SANG
Dept of Mechanical Enginrg
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