Glucagon-Secreting Alpha Cell Selective Two-Photon Fluorescent Probe TP-alpha: For Live Pancreatic Islet Imaging
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SCOPUS
- Title
- Glucagon-Secreting Alpha Cell Selective Two-Photon Fluorescent Probe TP-alpha: For Live Pancreatic Islet Imaging
- Authors
- Agrawalla, Bikram Keshari; Chandran, Yogeswari; Phue, Wut-Hmone; Lee, Sung-Chan; Jeong, Yun-Mi; Wan, Si Yan Diana; Kang, Nam-Young; Chang, Young-Tae
- Date Issued
- 2015-04
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Two-photon (TP) microscopy has an advantage for live tissue imaging which allows a deeper tissue penetration up to 1 mm comparing to one-photon (OP) microscopy. While there are several OP fluorescence probes in use for pancreatic islet imaging, TP imaging of selective cells in live islet still remains a challenge. Herein, we report the discovery of first TP live pancreatic islet imaging probe; TP-alpha (Two Photon-alpha) which can selectively stain glucagon secreting alpha cells. Through fluorescent image based screening using three pancreatic cell lines, we discovered TP-alpha from alpha TP fluorescent dye library TPG (TP-Green). In vitro fluorescence test showed that TP-alpha have direct interaction and appear glucagon with a significant fluorescence increase, but not with insulin or other hormones/analytes. Finally, TP-alpha was successfully applied for 3D imaging of live islets by staining alpha cell directly. The newly developed TP-alpha can be a practical tool to evaluate and identify live alpha cells in terms of localization, distribution and availability in the intact islets.
- Keywords
- BETA-CELLS; INSULIN-SECRETION; MOUSE; IDENTIFICATION; ABSORPTION; LANGERHANS; LIBRARY; DYE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50384
- DOI
- 10.1021/ja5115776
- ISSN
- 0002-7863
- Article Type
- Article
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 137, no. 16, page. 5355 - 5362, 2015-04
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