Discovery of a Structural-Element Specific G-Quadruplex "Light-Up" Probe
SCIE
SCOPUS
- Title
- Discovery of a Structural-Element Specific G-Quadruplex "Light-Up" Probe
- Authors
- Zhang, Liyun; Er, Jun Cheng; Ghosh, Krishna Kanta; Chung, Wan Jun; Yoo, Jaeduk; Xu, Wang; Zhao, Wei; Anh Tuan Phan; Chang, Young-Tae
- Date Issued
- 2014-01
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and four medium grooves. Mechanistic studies suggest that GQR associates with G-quadruplex grooves close to the end of the tetrad core, which may explain the dye's specificity to only a subset of parallel structures. This specific recognition favours the disaggregation of GQR in aqueous solutions thereby recovering the inherent fluorescence of the dye. Due to its unique features, GQR represents a valuable tool for basic biological research and the rapid discovery of novel, specific ligands that target similar structural features of G-quadruplexes.
- Keywords
- AGGREGATION-INDUCED EMISSION; FLUORESCENT-PROBE; DRUG-DELIVERY; DNA; OLIGONUCLEOTIDE; INTEGRASE; TELOMERES; STACKING; PROMOTER; SCAFFOLD
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50304
- DOI
- 10.1038/srep03776
- ISSN
- 2045-2322
- Article Type
- Article
- Citation
- SCIENTIFIC REPORTS, vol. 4, 2014-01
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- There are no files associated with this item.
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