Kinetics and Cellular Site of Glycolipid Loading Control the Outcome of Natural Killer T Cell Activation
SCIE
SCOPUS
- Title
- Kinetics and Cellular Site of Glycolipid Loading Control the Outcome of Natural Killer T Cell Activation
- Authors
- Im, Jin S.; Arora, Pooja; Bricard, Gabriel; Molano, Alberto; Venkataswamy, Manjunatha M.; Baine, Ian; Jerud, Elliot S.; Goldberg, Michael F.; Baena, Andres; Yu, Karl O. A.; Ndonye, Rachel M.; Howell, Amy R.; Yuan, Weiming; Cresswell, Peter; Chang, Young-tae; Illarionov, Petr A.; Besra, Gurdyal S.; Porcelli, Steven A.
- Date Issued
- 2009-06
- Publisher
- CELL PRESS
- Abstract
- CID1d-restricted natural killer T cells (NKT cells) possess a wide range of effector and regulatory activities that are related to their ability to secrete both T helper 1 (Th1) cell- and Th2 cell-type cytokines. We analyzed presentation of NKT cell activating a galactosylceramide (alpha GalCer) analogs that give predominantly Th2 cell-type cytokine responses to determine how ligand structure controls the outcome of NKT cell activation. Using a monoclonal antibody specific for alpha GalCer-CD1d complexes to visualize and quantitate glycolipid presentation, we found that Th2 cell-type cytokine-biasing ligands were characterized by rapid and direct loading of cell-surface CD1d proteins. Complexes formed by association of these Th2 cell-type cytokine-biasing alpha GalCer analogs with CD1d showed a distinctive exclusion from ganglioside-enriched, detergent-resistant plasma membrane microdomains of antigen-presenting cells. These findings help to explain how subtle alterations in glycolipid ligand structure can control the balance of proinflammatory and anti-inflammatory activities of NKT cells.
- Keywords
- HUMAN CD1D MOLECULES; LIGAND ALPHA-GALACTOSYLCERAMIDE; II-PEPTIDE COMPLEXES; NKT CELLS; PLASMA-MEMBRANE; DENDRITIC CELLS; LIPID RAFTS; IN-VIVO; RECOGNITION; PROTEINS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50235
- DOI
- 10.1016/j.immuni.2009.03.022
- ISSN
- 1074-7613
- Article Type
- Article
- Citation
- IMMUNITY, vol. 30, no. 6, page. 888 - 898, 2009-06
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