Ubiquitin C-terminal hydrolase 37 regulates Wnt/β-catenin pathway duing Xenopus gastrulation
- Title
- Ubiquitin C-terminal hydrolase 37 regulates Wnt/β-catenin pathway duing Xenopus gastrulation
- Authors
- HAN, JIN KWAN; HAN, JIN KWAN; LEE, HYEYOON
- Date Issued
- 2017-05-10
- Publisher
- JSDB
- Abstract
- TheTcf/LeffamilyoftranscriptionfactorsmediatestheWnt/β-cateninpathwaythatisinvolvedinawiderangeofbiologicalprocesses,includingvertebrateembryogenesisanddiversepathogenesis.Post-translationalmodifications,includingphosphorylation,sumoylationandacetylation,areknowntobeimportantfortheregulationofTcf/Lefproteins.However,theimportanceofubiquitinationandubiquitin-mediatedregulatorymechanismsforTcf/Lefactivityarestillunclear.Here,wenewlyshowthatubiquitinC-terminalhydrolase37(Uch37),adeubiquitinase,interactswithTcf7(formerlynamedTcf1)toactivateWntsignalling.BiochemicalanalysesdemonstratedthatdeubiquitinatingactivityofUch37isnotinvolvedinTcf7proteinstabilitybutisrequiredfortheassociationofTcf7totargetgenepromoterinbothXenopusembryoandhumanlivercancercells.InvivoanalysesfurtherrevealedthatUch37functionsasapositiveregulatoroftheWnt/β-cateninpathwaydownstreamofβ-cateninstabilizationthatisrequiredfortheexpressionofventrolateralmesodermgenesduringXenopusgastrulation.OurstudyprovidesanewmechanismforchromatinoccupancyofTcf7anduncoversthephysiologicalsignificanceofUch37duringearlyvertebratedevelopmentbyregulatingtheWnt/β-cateninpathway.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/43130
- Article Type
- Conference
- Citation
- 50TH ANNUAL MEETING OF THE JSDB 2017, 2017-05-10
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