Direct immobilization of antibodies on Zn-doped Fe3O4 nanoclusters for detection of pathogenic bacteria
SCIE
SCOPUS
- Title
- Direct immobilization of antibodies on Zn-doped Fe3O4 nanoclusters for detection of pathogenic bacteria
- Authors
- Monalisa Pal; LEE, SANG HEE; Donghoon Kwon; Jeongin Hwang; LEEHYEONJEONG; Seokyung Hwang; JEON, SANGMIN
- Date Issued
- 2017-02
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- Zinc-doped magnetic nanoclusters (Zn-MNCs) were synthesized and used to detect pathogenic bacteria in milk. Hydrothermally synthesized Zn-MNCs exhibited stronger magnetic properties than pure MNCs, which facilitated the magnetic separation from the sample using a permanent magnet. The presence of accessible Zn sites allows the direct immobilization of half-fragmented antibodies over Zn-MNCs through strong ZnS bonds and prevents the tedious multiple steps of molecular functionalization or coating with costly noble metals prior to conjugation with an antibody. After the capture and magnetic separation of Salmonella in milk using the antibody-functionalized Zn-MNCs, the concentration of bacteria was determined with a portable ATP luminometer and the detection limit was found to be 10 CFU/mL.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/41270
- DOI
- 10.1016/j.aca.2016.11.041
- ISSN
- 0003-2670
- Article Type
- Article
- Citation
- ANALYTICA CHIMICA ACTA, vol. 952, page. 81 - 87, 2017-02
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- There are no files associated with this item.
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