Ratiometric imaging of tissue by two-photon microscopy: Observation of a high level of formaldehyde around mouse intestinal crypts
SCIE
SCOPUS
- Title
- Ratiometric imaging of tissue by two-photon microscopy: Observation of a high level of formaldehyde around mouse intestinal crypts
- Authors
- SINGHA, SUBHANKAR; JUN, YONG WOONG; BAE, JURYANG; AHN, KYO HAN
- Date Issued
- 2017-03
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Ratiometric imaging by two-photon microscopy can offer a viable tool for the relative quantification of biological analytes inside tissue with minimal influence from environmental factors that affect fluorescence signal. We demonstrate the ratiometric imaging of formaldehyde at the suborgan level using a two-photon fluorescent
probe, which involves pixel-to-pixel ratiometric data transformation. This study reveals for the first time a high level of formaldehyde around the crypts of mouse small intestine, implicating its possible protective role along with the released antimicrobials from the Paneth cells.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/41258
- DOI
- 10.1021/acs.analchem.7b00044
- ISSN
- 0003-2700
- Article Type
- Article
- Citation
- ANALYTICAL CHEMISTRY, vol. 89, no. 6, page. 3724 - 3731, 2017-03
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.