Generating mammalian stable cell lines by electroporation
SCIE
SCOPUS
- Title
- Generating mammalian stable cell lines by electroporation
- Authors
- Longo, PA; Kavran, JM; Kim, MS; Leahy, DJ
- Date Issued
- 2013
- Publisher
- Elsevier
- Abstract
- Expression of functional, recombinant mammalian proteins often requires expression in mammalian cells (see Single Cell Cloning of a Stable Mammalian Cell Line). If the expressed protein needs to be made frequently, it can be best to generate a stable cell line instead of performing repeated transient transfections into mammalian cells. Here, we describe a method to generate stable cell lines via electroporation followed by selection steps. This protocol will be limited to the CHO dhfr - Urlaub et al. (1983) and LEC1 cell lines, which in our experience perform the best with this method.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/40816
- DOI
- 10.1016/B978-0-12-418687-3.00017-3
- ISSN
- 0076-6879
- Article Type
- Article
- Citation
- METHODS IN ENZYMOLOGY, vol. 529, page. 209 - 226, 2013
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- There are no files associated with this item.
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