'Deadman' and 'Passcode' microbial kill switches for bacterial containment
SCIE
SCOPUS
- Title
- 'Deadman' and 'Passcode' microbial kill switches for bacterial containment
- Authors
- Chan, Clement T. Y.; Lee, Jeong Wook; Cameron, D. Ewen; Bashor, Caleb J.; Collins, James J.
- Date Issued
- 2016-02
- Publisher
- Nature Publishing Group
- Abstract
- Biocontainment systems that couple environmental sensing with circuit-based control of cell viability could be used to prevent escape of genetically modified microbes into the environment. Here we present two engineered safeguard systems known as the `Deadman' and `Passcode' kill switches. The Deadman kill switch uses unbalanced reciprocal transcriptional repression to couple a specific input signal with cell survival. The Passcode kill switch uses a similar two-layered transcription design and incorporates hybrid LacI-GalR family transcription factors to provide diverse and complex environmental inputs to control circuit function. These synthetic gene circuits efficiently kill Escherichia coli and can be readily reprogrammed to change their environmental inputs, regulatory architecture and killing mechanism.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/39287
- DOI
- 10.1038/NCHEMBIO.1979
- ISSN
- 1552-4450
- Article Type
- Article
- Citation
- Nature Chemical Biology, vol. 12, no. 2, page. 82 - 86, 2016-02
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.