Nucleic acid detection with CRISPR-Cas13a/C2c2
SCOPUS
- Title
- Nucleic acid detection with CRISPR-Cas13a/C2c2
- Authors
- Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Lee, Jeong Wook; Essletzbichler, Patrick; Dy, Aaron J.; Joung, Julia; Verdine, Vanessa; Donghia, Nina; Daringer, Nicole M.; Freije, Catherine A.; Myhrvold, Cameron; Bhattacharyya, Roby P.; Livny, Jonathan; Regev, Aviv; Koonin, Eugene V.; Hung, Deborah T.; Sabeti, Pardis C.; Collins, James J.; Zhang, Feng
- Date Issued
- 2017-04-28
- Publisher
- AAAS
- Abstract
- Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumor DNA. Furthermore, SHERLOCK reaction reagents can be lyophilized for cold-chain independence and long-term storage and be readily reconstituted on paper for field applications.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/39148
- DOI
- 10.1126/science.aam9321
- ISSN
- 0036-8075
- Article Type
- Article
- Citation
- Science, vol. 356, no. 6336, page. 438 - 442, 2017-04-28
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- There are no files associated with this item.
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