Intracellular Delivery of Bioactive Cargos to Hard-to-Transfect Cells Using Carbon Nanosyringe Arrays under an Applied Centrifugal g-Force
SCIE
SCOPUS
- Title
- Intracellular Delivery of Bioactive Cargos to Hard-to-Transfect Cells Using Carbon Nanosyringe Arrays under an Applied Centrifugal g-Force
- Authors
- Minsuk Choi; Sang Ho Lee; Kim, WB; Vipul Gujrati; Daejin Kim; Jinju Lee; Jae-Il Kim; Hyungjun Kim; Phei Er Saw; Sangyong Jon
- Date Issued
- 2016-01
- Publisher
- Wiley
- Abstract
- There is considerable interest in developing a common, universal platform for delivering biomacromolecules such as proteins and RNAs into diverse cells with high efficiency. Here, it is shown that carbon nanosyringe arrays (CNSAs) under an applied centrifugal g-force (cf-CNSAs) can deliver diverse bioactive cargos directly into the cytosol of hard-to-transfect cells with relatively high efficiency and reproducibility. The cf-CNSA platform, an optimized version of a previous CNSA-mediated intracellular delivery platform that adds a g-force feature, exhibits more rapid and superior delivery of cargos to various hard-to-transfect cells than is the case in the absence of g-force. Active species, including small interfering RNAs, plasmids, and proteins are successfully transported across plasma membrane barriers into various cells. By overcoming the limitations of currently available transfection methods, the cf-CNSA platform paves the way to universal delivery of a variety of cargos, facilitating the analysis of cellular responses in diverse cell types.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37913
- DOI
- 10.1002/ADHM.201400834
- ISSN
- 2192-2640
- Article Type
- Article
- Citation
- Advanced Healthcare Materials, vol. 5, no. 1, page. 101 - 107, 2016-01
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- There are no files associated with this item.
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