High-precision THz Dielectric Spectroscopy of Tris-HCl Buffer
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- Title
- High-precision THz Dielectric Spectroscopy of Tris-HCl Buffer
- Authors
- Lee, S; Kang, H; Do, Y; Lee, G; KIM, JIN WOO; HAN, HAEWOOK
- Date Issued
- 2016-06
- Publisher
- Optical Society of Korea
- Abstract
- Tris-HCl buffer solution is extensively used in biochemistry and molecular biology to maintain a stablepH for biomolecules such as nucleic acids and proteins. Here we report on the high-precision THz dielectricspectroscopy of a 10 mM Tris-HCl buffer. Using a double Debye model, including conductivity of ionicspecies, we measured the complex dielectric functions of Tris-HCl buffer. The fast relaxation time of watermolecules in Tris-HCl buffer is ~20% longer than that in pure water while the slow relaxation time changeslittle. This means that the reorientation dynamics of Tris-HCl buffer with such a low Tris concentrationis quite different from that of pure water.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37799
- DOI
- 10.3807/JOSK.2016.20.3.431
- ISSN
- 1226-4776
- Article Type
- Article
- Citation
- Journal of the Optical Society of Korea, vol. 20, no. 3, page. 431 - 434, 2016-06
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