In Vitro Selection of pH‐Activated DNA Nanostructures
SCIE
SCOPUS
- Title
- In Vitro Selection of pH‐Activated DNA Nanostructures
- Authors
- Fong, FY; Oh, SS; Hawker, CJ; Soh, HT
- Date Issued
- 2016-12-05
- Publisher
- Wiley-VCH
- Abstract
- We report the first in vitro selection of DNA nanostructures that switch their conformation when triggered by change in pH. Previously, most pH-active nanostructures were designed using known pH-active motifs, such as the i-motif or the triplex structure. In contrast, we performed de novo selections starting from a random library and generated nanostructures that can sequester and release Mipomersen, a clinically approved antisense DNA drug, in response to pH change. We demonstrate extraordinary pH-selectivity, releasing up to 714-fold more Mipomersen at pH5.2 compared to pH7.5. Interestingly, none of our nanostructures showed significant sequence similarity to known pH-sensitive motifs, suggesting that they may operate via novel structure-switching mechanisms. We believe our selection scheme is general and could be adopted for generating DNA nanostructures for many applications including drug delivery, sensors and pH-active surfaces.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37677
- DOI
- 10.1002/ANIE.201607540
- ISSN
- 1433-7851
- Article Type
- Article
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, vol. 55, no. 49, page. 15258 - 15262, 2016-12-05
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