Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy
SCIE
SCOPUS
- Title
- Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy
- Authors
- Min Jung Yang; Sook Hee Ku; Dongkyu Kim; Kim, WJ; Hyejung Mok; Sun Hwa Kim; Ick Chan Kwon
- Date Issued
- 2015-12
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- This study aims to develop bioreducible polyethylenimine (rPEI)/siRNA polyplexes with high stability, high transfection efficiency, and low cytotoxicity for efficient cytoplasmic siRNA delivery. rPEI successfully incorporated siRNA into stable and compact nano-complexes, and the disulfide linkages in rPEI/siRNA were cleaved under reductive environments, resulting in efficient intracellular translocation and siRNA release. In this study, receptor for advanced glycation end-products (RAGE) was selected as a therapeutic target gene because it is associated with inflammatory responses in ischemia/reperfusion injury. rPEI/siRAGE exhibited high target gene silencing and low cytotoxicity in cardiomyocytes, and the treatment of rPEI/siRAGE reduced the myocardial infarction size.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37473
- DOI
- 10.1002/MABI.201500213
- ISSN
- 1616-5187
- Article Type
- Article
- Citation
- MACROMOLECULAR BIOSCIENCE, vol. 15, no. 12, page. 1755 - 1763, 2015-12
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- There are no files associated with this item.
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