Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants
SCIE
SCOPUS
- Title
- Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants
- Authors
- Oh, YJ; Kim, H; Seo, SH; Hwang, BG; CHANG, YOON SEOK; Lee, J; Lee, DW; Sohn, EJ; LEE, SANG JOON; LEE, YOUNGSOOK; Hwang, I
- Date Issued
- 2016-04-04
- Publisher
- CELL PRESS
- Abstract
- Rhizosphere acidification is essential for iron (Fe) uptake into plant roots. Plasma membrane (PM) H+-ATPases play key roles in rhizosphere acidification. However, it is not fully understood how PM H+-ATPase activity is regulated to enhance root Fe uptake under Fe-deficient conditions. Here, we present evidence that cytochrome b5 reductase 1 (CBR1) increases the levels of unsaturated fatty acids, which stimulate PM H+-ATPase activity and thus lead to rhizosphere acidification. CBR1-overexpressing (CBR1-OX) Arabidopsis thaliana plants had higher levels of unsaturated fatty acids (18: 2 and 18: 3), higher PM H+-ATPase activity, and lower rhizosphere pH than wild-type plants. By contrast, cbr1 loss-of-function mutant plants showed lower levels of unsaturated fatty acids and lower PM H+-ATPase activity but higher rhizosphere pH. Reduced PM H+-ATPase activity in cbr1 could be restored in vitro by addition of unsaturated fatty acids. Transcript levels of CBR1, fatty acids desaturase 2 (FAD2), and fatty acids desaturase 3 (FAD3) were increased under Fe-deficient conditions. We propose that CBR1 has a crucial role in increasing the levels of unsaturated fatty acids, which activate the PM H+-ATPase and thus reduce rhizosphere pH. This reaction cascade ultimately promotes root Fe uptake.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37080
- DOI
- 10.1016/J.MOLP.2015.12.010
- ISSN
- 1674-2052
- Article Type
- Article
- Citation
- Molecular plant, vol. 9, no. 4, page. 501 - 1674-2052, 2016-04-04
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.