Non-contact high-frequency ultrasound microbeam stimulation for studying mechanotransduction in human umbilical vein endothelial cells
SCIE
SCOPUS
- Title
- Non-contact high-frequency ultrasound microbeam stimulation for studying mechanotransduction in human umbilical vein endothelial cells
- Authors
- Hwang, JY; LIM, HAEGYUN; Yoon, CW; Lam, KH; Yoon, S; Lee, C; Chiu, CT; Kang, BJ; Kim, HH; Shung, KK
- Date Issued
- 2014-09
- Publisher
- IEEE Trans
- Abstract
- We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechano-transduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. Avariety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified. (E-mail: jyhwang@dgist.ac.kr) (C) 2014 World Federation for Ultrasound in Medicine & Biology.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36627
- DOI
- 10.1016/J.ULTRASMEDBIO.2014.03.018
- ISSN
- 0301-5629
- Article Type
- Article
- Citation
- Ultrasound in Medicine and Biology, vol. 40, no. 9, page. 2172 - 2182, 2014-09
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- There are no files associated with this item.
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