Enhanced cellular distribution and infiltration in a wet electrospun three-dimensional fibrous scaffold using eccentric rotation-based hydrodynamic conditions
SCIE
SCOPUS
- Title
- Enhanced cellular distribution and infiltration in a wet electrospun three-dimensional fibrous scaffold using eccentric rotation-based hydrodynamic conditions
- Authors
- Joonseong Heo; Hyoryung Nam; Dongha Hwang; Seong J. Cho; Sung-Young Jung; Cho, DW; Jin-Hyung Shim; Lim, G
- Date Issued
- 2016-04
- Publisher
- ELSEVIER
- Abstract
- Electrospinning technology is a versatile method for fabricating three-dimensional (3D) nanofibrous scaffolds using a wide range of polymeric materials for tissue engineering and regenerative medicine. However, a major concern regarding 3D electrospun scaffolds is that the densely packed layers hinder an even cellular distribution and in-depth infiltration. Here, we describe a new 'all-at-once' method enabling scaffold fabrication and cell seeding simultaneously, in which the medium bath containing cells is rotated eccentrically at high speed (>1500 rpm). The unstable flow of culture medium under hydrodynamic conditions resulted in a skein-shaped 3D structure and enhanced the even cellular distribution and in-depth infiltration. Cellular distribution and infiltration analyses confirmed that our method was superior to static and dynamic seeding methods. Moreover, we showed that our method facilitated long-term (14 days) proliferation. (C) 2015 Elsevier B.V. All rights reserved.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36518
- DOI
- 10.1016/J.SNB.2015.11.030
- ISSN
- 0925-4005
- Article Type
- Article
- Citation
- Sensors & Actuators B: Chemical, vol. 226, page. 357 - 363, 2016-04
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