Quantification of Fewer than Ten Copies of a DNA Biomarker without Amplification or Labeling
SCIE
SCOPUS
- Title
- Quantification of Fewer than Ten Copies of a DNA Biomarker without Amplification or Labeling
- Authors
- Lee, Y; Kim, Y; Lee, D; Roy, D; Park, JW
- Date Issued
- 2016-06-08
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Polymerase chain reaction (PCR) is a highly sensitive diagnosis technique for detection of nucleic acids and for monitoring residual disease; however, PCR can be unreliable for samples containing very few target molecules. Here, we describe a quantification method, using force distance (FD) curve based atomic force microscopy (AFM) to detect a target DNA bound to small (1.4-1.9 mu m diameter) probe DNA spots, allowing mapping of entire spots to nanometer resolution. Using a synthetic BCR-ABL fusion gene sequence target, we examined samples containing between one and 10 target copies. A high degree of correlation (r(2) = 0.994) between numbers of target copies and detected probe clusters was observed, and the approach could detect the BCR-ABL biomarker when only a single copy was present, although multiple screens were required. Our results dearly demonstrate that FD curve-based imaging is suitable for quantitative analysis of fewer than 10 copies of DNA biomarkers without amplification, modification, or labeling.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36462
- DOI
- 10.1021/JACS.6B02791
- ISSN
- 0002-7863
- Article Type
- Article
- Citation
- JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 138, no. 22, page. 7075 - 7081, 2016-06-08
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