Noninvasive Transdermal Vaccination Using Hyaluronan Nanocarriers and Laser Adjuvant
SCIE
SCOPUS
- Title
- Noninvasive Transdermal Vaccination Using Hyaluronan Nanocarriers and Laser Adjuvant
- Authors
- Kim, KS; Kim, H; Park, Y; Kong, WH; LEE, SEUNG WOO; Kwok, SJJ; Hahn, SK; Yun, SH
- Date Issued
- 2016-04-19
- Publisher
- Wiley Online Library
- Abstract
- Vaccines are commonly administered by injection using needles. Although transdermal microneedles are less invasive promising alternatives, needle-free topical vaccination without involving physical damage to the natural skin barrier is still sought after as it can further reduce needle-induced anxiety and is simple to administer. However, this long-standing goal has been elusive since the intact skin is impermeable to most macromolecules. Here, we show an efficient, noninvasive transdermal vaccination by employing two key innovations: the use of hyaluronan (HA) as vaccine carriers and non-ablative laser adjuvants. Conjugates of a model vaccine ovalbumin (OVA) and HAHA-OVA conjugates-induced more effective maturation of dendritic cells in vitro, compared to OVA. Following topical administration in the skin, HA-OVA conjugates penetrated into the epidermis and dermis in murine and porcine skins, as revealed by intravital microscopy and fluorescence assay. Topical administration of HA-OVA conjugates significantly elevated both humoral and mucosal antibodies, with peak levels at four weeks. An OVA challenge at week eight elicited strong immune-recall responses. With pretreatment of the skin using non-ablative fractional laser beams as adjuvant, strong immunization was achieved with much reduced doses of HA-OVA (1 mg kg(-1) OVA). Our results demonstrate the potential of the noninvasive patch-type transdermal vaccination platform.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36446
- DOI
- 10.1002/ADFM.201504879
- ISSN
- 1616-301X
- Article Type
- Article
- Citation
- Advanced Functional Materials, vol. 26, no. 15, page. 2512 - 2522, 2016-04-19
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.