An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering
SCIE
SCOPUS
- Title
- An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering
- Authors
- Kundu, J; Shim, JH; JANG, JINAH; Kim, SW; Cho, DW
- Date Issued
- 2015-11
- Publisher
- WILEY-BLACKWELL
- Abstract
- Regenerative medicine is targeted to improve, restore or replace damaged tissues or organs using a combination of cells, materials and growth factors. Both tissue engineering and developmental biology currently deal with the process of tissue self-assembly and extracellular matrix (ECM) deposition. In this investigation, additive manufacturing (AM) with a multihead deposition system (MHDS) was used to fabricate three-dimensional (3D) cell-printed scaffolds using layer-by-layer (LBL) deposition of polycaprolactone (PCL) and chondrocyte cell-encapsulated alginate hydrogel. Appropriate cell dispensing conditions and optimum alginate concentrations for maintaining cell viability were determined. In vitro cell-based biochemical assays were performed to determine glycosaminoglycans (GAGs), DNA and total collagen contents from different PCL-alginate gel constructs. PCL-alginate gels containing transforming growth factor-(TGF) showed higher ECM formation. The 3D cell-printed scaffolds of PCL-alginate gel were implanted in the dorsal subcutaneous spaces of female nude mice. Histochemical [Alcian blue and haematoxylin and eosin (H&E) staining] and immunohistochemical (type II collagen) analyses of the retrieved implants after 4weeks revealed enhanced cartilage tissue and type II collagen fibril formation in the PCL-alginate gel (+TGF) hybrid scaffold. In conclusion, we present an innovative cell-printed scaffold for cartilage regeneration fabricated by an advanced bioprinting technology. Copyright (C) 2013 John Wiley & Sons, Ltd.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36194
- DOI
- 10.1002/TERM.1682
- ISSN
- 1932-6254
- Article Type
- Article
- Citation
- JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol. 9, no. 11, page. 1286 - 1297, 2015-11
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