Functional characterization of Vibrio cholerae O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis
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- Title
- Functional characterization of Vibrio cholerae O1 WbeW enzyme responsible for initial reaction in O antigen biosynthesis
- Authors
- Kim, CS; Seo, JH; Cha, HJ
- Date Issued
- 2015-11
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Abstract
- Vibrio cholerae O1 employs the ATP-binding cassette (ABC) transporter-dependent pathway for O antigen biosynthesis. Different from highly studied Klebsiella pneumoniae and Escherichia coli, it was reported that initial reaction of O antigen biosynthesis in V. cholerae O1 may be involved in WbeW protein, which is predicted to be a galactosyltransferase. In this work, we report expression and characterization of WbeW enzyme. WbeW was expressed as membrane-associated form in E. coli and it was obtained with high purity. The enzyme had a function of transferring Gal-1-P from UDP-Gal to Und-P, implying that initial glycan of O antigen in V. cholerae O1 can be composed of a Gal residue.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36181
- DOI
- 10.1007/S12257-015-0677-9
- ISSN
- 1226-8372
- Article Type
- Article
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, vol. 20, no. 6, page. 980 - 987, 2015-11
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