Rapid Communication Biomimetic Repeat Protein Derived from Xenopus Tropicalis for Fibrous Scaffold Fabrication
SCIE
SCOPUS
- Title
- Rapid Communication Biomimetic Repeat Protein Derived from Xenopus Tropicalis for Fibrous Scaffold Fabrication
- Authors
- Kwon, Y; Yang, YJ; Jung, D; Hwang, BH; Cha, HJ
- Date Issued
- 2015-12
- Publisher
- WILEY-BLACKWELL
- Abstract
- Collagen, silk, and elastin are the fibrous proteins consist of representative amino acid repeats. Because these proteins exhibited distinguishing mechanical properties, they have been utilized in diverse applications, such as fiber-based sensors, filtration membranes, supporting materials, and tissue engineering scaffolds. Despite their infinite prevalence and potential, most studies have only focused on a few repeat proteins. In this work, the hypothetical protein with a repeat motif derived from the frog Xenopus tropicalis was obtained and characterized for its potential as a novel protein-based material. The codon-optimized recombinant frog repeat protein, referred to as 'xetro', was produced at a high rate in a bacterial system, and an acid extraction-based purified xetro protein was successfully fabricated into microfibers and nanofibers using wet spinning and electrospinning, respectively. Specifically, the wet-spun xetro microfibers demonstrated about 2-and 1.5-fold higher tensile strength compared with synthetic polymer polylactic acid and cross-linked collagen, respectively. In addition, the wet-spun xetro microfibers showed about sevenfold greater stiffness than collagen. Therefore, the mass production potential and greater mechanical properties of the xetro fiber may result in these fibers becoming a new promising fiber-based material for biomedical engineering. (C) 2015 Wiley Periodicals, Inc.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/36179
- DOI
- 10.1002/BIP.22735
- ISSN
- 0006-3525
- Article Type
- Article
- Citation
- BIOPOLYMERS, vol. 103, no. 12, page. 659 - 664, 2015-12
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