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High-yield isolation of extracellular vesicles using aqueous two-phase system SCIE SCOPUS

Title
High-yield isolation of extracellular vesicles using aqueous two-phase system
Authors
Shin, HHan, CLabuz, JMKim, JKim, JCho, SGho, YSTakayama, SPark, J
Date Issued
2015-08-14
Publisher
Nature Publishing Group
Abstract
Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (similar to 70%) in a short time (similar to 15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.
URI
https://oasis.postech.ac.kr/handle/2014.oak/35602
DOI
10.1038/SREP13103
ISSN
2045-2322
Article Type
Article
Citation
Scientific Reports, vol. 5, 2015-08-14
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박재성PARK, JAE SUNG
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