Impaired IL-2 expression in latent HIV-1 infection
SCIE
SCOPUS
- Title
- Impaired IL-2 expression in latent HIV-1 infection
- Authors
- Shin, Y; Yoon, CH; Lim, H; Park, J; Roh, TY; Kang, C; Choi, BS
- Date Issued
- 2015-08-07
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- Regarding the T cell function in HIV-1 infection, activation of T cells is enhanced in acutely HIV-1-infected T cells upon stimuli. However, T cell immune responses underlying the activation of T cell receptor (TCR) signaling molecules and interleukin (IL)-2 production in latently HIV-1-infected cells are poorly understood. The expression and activation of TCR components and its downstream molecules in acutely and latently HIV-1-infected T cells were compared using quantitative reverse transcription polymerase chain reaction (RT-PCR) for mRNA expression and enzyme-linked immunosorbent assay (ELISA) for levels of IL-2 in phytohemagglutinin M (PHA-M). The levels of T cell surface molecules and TCR signaling molecules in latently HIV-1-infected cells were greatly decreased without changes in their mRNA levels. In addition, downstream TCR-signaling molecules in latently HIV-1-infected cells were not activated even in the presence of PHA-M. The phosphorylation of mitogen-activated protein kinases (MAPKs) in the presence of PHA-M was weakly induced in latently HIV-1-infected cells but was greater in acutely HIVNL4-3-infected cells. Finally, the production of IL-2 was significantly decreased in latently HIV-1-infected cells compared with uninfected parent cells. Thus, IL-2-related immunological functions in latently HIV-1-infected T cells were markedly impaired even in the presence of stimuli. (C) 2015 Elsevier Inc. All rights reserved.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/35590
- DOI
- 10.1016/J.BBRC.2015.06.091
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 463, no. 4, page. 1237 - 1242, 2015-08-07
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